clock device (plux/tet) (+luxI, +tetR, +aiiA, ++sRNA, +amilGFP, +UV reset device(E234K, -LVA)
The full construct for Multicellular Analog Clock.
LuxI and LuxR forms the positive feedback loop of AHL production. TetR, AiiA, and sRNAs are the suppressors for negative feedback loops. mCherry is the red reporter protein for visualization of the excitation wave (gclock handh). amilGFP is the yellow reporter protein expressed constitutively as an internal standard. cI repressor and PR promoter forms UV reset device to make refractory period with UV induction.
You can make the Multicellular Analog Clock with this BioBrick part (BBa_K1124100). The excition wave of gene expression are generated on an agar plate and visualized for naked eyes.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 6228
Illegal NheI site found at 6251
Illegal NheI site found at 7215
Illegal NheI site found at 7238
Illegal NheI site found at 8159
Illegal NheI site found at 8182
- 21Illegal BglII site found at 4519
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 885
Illegal AgeI site found at 5361
- 1000Illegal BsaI.rc site found at 2353
Illegal BsaI.rc site found at 2681
Illegal BsaI.rc site found at 3009