Composite

Part:BBa_K112363

Designed by: Aron Lau   Group: iGEM08_UC_Berkeley   (2008-10-28)


{Pbad}{rbs_lamB>} {< r.BglII>}{< phoA!}{< b1006>}

{Pbad}{rbs_lamB>} {< r.BglII>}{< phoA!}{< b1006>}

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 33
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 33
    Illegal NheI site found at 1184
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 33
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 33
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 33
    Illegal NgoMIV site found at 2208
    Illegal NgoMIV site found at 2655
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1050


[edit]
Categories
Parameters
None