Composite

Part:BBa_K112361

Designed by: Aron Lau   Group: iGEM08_UC_Berkeley   (2008-10-28)


{Pbad}{rbs_pelB>} {< ligase>}{< phoA!}{< b1006>}

{Pbad}{rbs_pelB>} {< ligase>}{< phoA!}{< b1006>}

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 33
    Illegal XbaI site found at 2627
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 33
    Illegal NheI site found at 1184
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 33
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 33
    Illegal XbaI site found at 2627
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 33
    Illegal XbaI site found at 2627
    Illegal NgoMIV site found at 1304
    Illegal NgoMIV site found at 2991
    Illegal NgoMIV site found at 3438
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1651
    Illegal SapI site found at 1050


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Categories
Parameters
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