Translational_Unit

Part:BBa_K1122679

Designed by: Aleksandra Lewicka, Jan Lyczakowski   Group: iGEM13_Edinburgh   (2013-08-30)


pSpac - LacZ - fused pdc and adhB

This translational unit enables expression of pyruvate decarboxylase (pdc) and alcohol dehydrogenase B (adhB) fused peptides in Bacillus subtilis using xylose induction. Production of pdc and adhB fused enzymes allows for conversion of pyruvate to ethanol via an acetaldehyde intermediate. The fusion state generates increased ethanol yeilds in aerobic conditions what should have a beneficial effect on EtOH production in B. subtilis

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 899
    Illegal AgeI site found at 2105
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None