Coding
EntF

Part:BBa_K1122667

Designed by: Kyle Rothschild-Mancinelli   Group: iGEM13_Edinburgh   (2013-07-29)


Enterobactin synthase component F

Activates the carboxylate group of L-serine via ATP-dependent PPi exchange reactions to the aminoacyladenylate, preparing that molecule for the final stages of enterobactin synthesis. Holo-EntF acts as the catalyst for the formation of the three amide and three ester bonds present in the cyclic (2,3-dihydroxybenzoyl)serine trimer enterobactin, using seryladenylate and acyl-holo-EntB (acylated with 2,3-dihydroxybenzoate by EntE).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 841
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 841
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 841
    Illegal BglII site found at 459
    Illegal BamHI site found at 1404
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 841
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 841
    Illegal AgeI site found at 1252
    Illegal AgeI site found at 2830
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
None