Plasmid

Part:BBa_K112247

Designed by: Molly Allen   Group: iGEM08_UC_Berkeley   (2008-10-28)


{pAH57 promoter}{rbs.xis.int!} inserted into K112950 plasmid

This part is inserted just before the attL1 site in this plasmid.

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2429
    Illegal XbaI site found at 3672
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2429
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 2429
    Illegal BamHI site found at 3339
    Illegal XhoI site found at 4825
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2429
    Illegal XbaI site found at 3672
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2429
    Illegal XbaI site found at 3672
    Illegal NgoMIV site found at 4678
    Illegal AgeI site found at 3077
    Illegal AgeI site found at 3189
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination/lambda
Parameters
None