Part:BBa_K112246

{pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112995 plasmid

All ccdb parts have been withdrawn from the Registry. Samples of parts containing the ccdB gene cannot be requested. - iGEM HQ

This part is inserted into the K112995 plasmid between ccdB and attR2.

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 3854
  Illegal PstI site found at 881
  Illegal PstI site found at 5837
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 4696
  Illegal PstI site found at 881
  Illegal PstI site found at 5837
  Illegal NotI site found at 141
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 212
  Illegal XhoI site found at 5877
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 3854
  Illegal PstI site found at 881
  Illegal PstI site found at 5837
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 3854
  Illegal PstI site found at 881
  Illegal PstI site found at 5837
  Illegal AgeI site found at 4286
  Illegal AgeI site found at 4610
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 762
  Illegal BsaI.rc site found at 4978