CMV promoter - GFP - hGH polyA tail
This is a mammalian GFP generator used for characterization of our CMV promoter (BBa_K1119006). This GFP generator contains the CMV promoter sequence with GFP reporter in RFC25 format(BBa_K648013) and hGH polyA terminator (BBa_K404108).
This GFP generator was then transfected into HEK293FT cells and in vivo green fluorescence signal was observed under confocal microscope.
pEGFP-N1 (Clontech) served as the positive control. It contains the CMV promoter and EGFP reporter. A GFP generator that does not contain the CMV promoter was used as the negative control.
The detailed protocol of our characterization can be found in HKUST iGEM 2013 Wiki.
iGEM Technion 2017 improved this part to BBa_K2520006. This improved device contains a TRE promoter instead of the original CMV, to allow for inducible expression of GFP within mammalian cells.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 614
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 628
Illegal AgeI site found at 1369
- 1000Illegal BsaI site found at 1772
Illegal BsaI.rc site found at 1274