aceB codes for malate synthase, native in E. coli K-12 strain BW25113. This part is useful for regulating metabolic pathway. It encodes an enzyme involved in the glyoxylate cycle in plants and bacteria. The glyoxylate enzyme allows plants and bacteria to use fatty acids as a substrate for gluconeogenesis and avoid complete oxiation into carbon dioxide. HKUST 2013 team has introduced this prokaryotic gene into mammalian cell to create an artificial futile cycle that increases uptake of fatty acids in the medium.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BamHI site found at 1382
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 1416
- 1000Illegal SapI.rc site found at 1015