Coding

Part:BBa_K1117004

Designed by:   Group: iGEM13_BIT-China   (2013-09-15)

ThiF

ThiF acts like an E1 ubiquitin-activating enzyme for ThiS, which is a ubiquitin-like protein in prokaryotes. The process of ubiquitination can target denatured proteins and stimulate their degradation.

We verified the function of ThiF by observing the growth of E.coli at 40℃，43℃ and 46℃.

BIT-China-ThiF-2.jpg Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None