Composite

Part:BBa_K1116005

Designed by: Shuguang Peng   Group: iGEM13_Tsinghua-A   (2013-09-11)


CAG-Cerulean-hsa-miR-21

In our project,the plasmid functions as a input ,which produce miRNA21 targets on T21.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1706
    Illegal XbaI site found at 1610
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1706
    Illegal NotI site found at 2477
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1706
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1706
    Illegal XbaI site found at 1610
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1706
    Illegal XbaI site found at 1610
    Illegal NgoMIV site found at 1298
    Illegal NgoMIV site found at 1455
    Illegal AgeI site found at 1744
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None