Composite

Part:BBa_K1116003

Designed by: Shuguang Peng   Group: iGEM13_Tsinghua-A   (2013-09-10)


Tre-LacI with miR-FF5 and miR-FF3 target

It serve as a intermediate in control circuit.It is induced by rtta from upstream plasmid and can repress the CAG promoter.It is also influenced by miR-FF3.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 381
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 381
    Illegal NotI site found at 1544
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 381
    Illegal BamHI site found at 343
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 381
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 381
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None