Translational_Unit
Part:BBa_K1115007
Designed by: Andrew Tuckwell Group: iGEM13_SydneyUni_Australia (2013-09-17)
dhlB 2-haloalkanoic acid dehalogenase
dhlB 2-haloalkanoic acid dehalogenase (dhlB, UniprotKB: [http://www.uniprot.org/uniprot/Q60099 Q60099]),initially identified in Xantobacter autotrophicus strain GJ10, catalyses the fourth step of 1-2-dichloroethane (DCA) degredation: hydrolysis of chloroacetic acid (chloracetate) to glyolic acid (glycolate)[1] .
dhlB shows activity on other halogenated alkanes including (but not limited to): mono- and di-chloroacetates, mono-and di-bromoacetates and chloropropinates. [2]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 267
Illegal NgoMIV site found at 509 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 56
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Categories
Parameters
None |