Reporter
Part:BBa_K1114700
Designed by: Jake Awtry Group: iGEM13_BostonU (2013-09-17)
Level 1 MoClo device that constitutively expresses mCitrine
This is a BioBrick adaptation of a Level 1 MoClo device that constitutively expresses the fluorescent protein mCitrine. It contains the Level 0 parts J23104_EB, BCD2_BC, mCitrine_CD, and B0015_DE within the pSB1C3 backbone. In the given sequence the internal fusion sites are incorrectly duplicated. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |