Composite

Part:BBa_K1114521

Designed by: Shawn Jin   Group: iGEM13_BostonU   (2013-09-13)


QS2

Transcriptional 2 of the CivI CivR system for Ecoli.

Includes. J23104-BCD2-CivR-B0015 these are flanked by the EF fusion site in a KAN backbone.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 195
    Illegal NgoMIV site found at 722
    Illegal AgeI site found at 476
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 489


[edit]
Categories
Parameters
None