Reporter
Part:BBa_K1114505
Designed by: Jake Awtry Group: iGEM13_BostonU (2013-09-12)
MoClo Level 1 RFP reporter with AE fusion sites
pJ04B16Rm_AE This is a MoClo Level 1 reporting device containing the Level 0 parts J23104_AB, BCD16_BC, E1010m_CD, and B0015_DE within the DVL1_AE backbone. The flanking fusion sites are A on the 5' side and E on the 3' side of the insert. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. In the given sequence the internal fusion sites are incorrectly duplicated. See Level 0 pages for further information.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1238
Illegal XbaI site found at 1265
Illegal SpeI site found at 994
Illegal PstI site found at 1277 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1238
Illegal NheI site found at 11
Illegal NheI site found at 34
Illegal SpeI site found at 994
Illegal PstI site found at 1277 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1238
Illegal BamHI site found at 1259 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1238
Illegal XbaI site found at 1265
Illegal SpeI site found at 994
Illegal PstI site found at 1277 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1238
Illegal XbaI site found at 1265
Illegal SpeI site found at 994
Illegal PstI site found at 1277
Illegal AgeI site found at 710
Illegal AgeI site found at 822 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1502
Illegal BsaI.rc site found at 1014
[edit]
Categories
Parameters
None |