Coding

Part:BBa_K1114413

Designed by: Shawn Jin   Group: iGEM13_BostonU   (2013-09-07)


DVL0_GB

LacZ Alpha with GB Fusion site

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 245
    Illegal XbaI site found at 272
    Illegal SpeI site found at 1
    Illegal SpeI site found at 529
    Illegal PstI site found at 284
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 245
    Illegal SpeI site found at 1
    Illegal SpeI site found at 529
    Illegal PstI site found at 284
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 245
    Illegal BamHI site found at 266
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 245
    Illegal XbaI site found at 272
    Illegal SpeI site found at 1
    Illegal SpeI site found at 529
    Illegal PstI site found at 284
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 245
    Illegal XbaI site found at 272
    Illegal SpeI site found at 1
    Illegal SpeI site found at 529
    Illegal PstI site found at 284
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 8
    Illegal BsaI.rc site found at 522


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Parameters
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