Reporter

Part:BBa_K1114207

Designed by: Shawn Jin   Group: iGEM13_BostonU   (2013-09-07)

CyPet_CD

This is a MoClo formatted CyPet fluorescent reporter gene with CD fusion sites.

This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Usage and Biology


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 218
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 218
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 218
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 648

Source

AddGene plasmid #14030; derived from FRET proteins


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Categories
Parameters
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