Coding

Part:BBa_K1114206

Designed by: Shawn Jin   Group: iGEM13_BostonU   (2013-09-07)

CviR_CD


This is a MoClo formatted CviR gene with CD fusion sites. This is a LuxR-homologue. CviR is the repressor gene in quorum sensing in Chromobacterium violaceum.

This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.


Source

Chromobacterium violaceum strain ATCC 12472 genomic DNA, obtained from the Holden Lab at the University of Wisconsin-Madison

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 44
    Illegal NgoMIV site found at 571
    Illegal AgeI site found at 325
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 338


[edit]
Categories
Parameters
None