Coding

Part:BBa_K1114203

Designed by: Shawn Jin   Group: iGEM13_BostonU   (2013-09-07)

C0071_CD Moclo of BBA_C0071

This is a MoClo formatted C0071 rhlR gene with CD fusion sites.

This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Usage and Biology


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 719

Source

iGEM distribution kit 2013


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