Coding
Part:BBa_K1114203
Designed by: Shawn Jin Group: iGEM13_BostonU (2013-09-07)
C0071_CD Moclo of BBA_C0071
This is a MoClo formatted C0071 rhlR gene with CD fusion sites.
This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Usage and Biology
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 244
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 719
Source
iGEM distribution kit 2013
[edit]
Categories
Parameters
None |