Coding

Part:BBa_K1114200

Designed by: Shawn Jin   Group: iGEM13_BostonU   (2013-09-07)

aaDa_CD

This is a MoClo formatted spectinimycin resistance gene with CD fusion sites.

This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Usage and Biology


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 649
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

Genomic DNA from E. coli K12 MG1655


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Parameters
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