Reporter

Part:BBa_K1113600

Designed by: Sebastián Alvarez   Group: iGEM13_UC_Chile   (2013-09-16)

GFP with a targeting sequence for delivery into the Carboxysome and a ssrA degradation tag.

This part consist in the superfolder GFP (BBa_I746916) attached to two targeting sequences: The large subunit of the ribulose-1,5-bisphosphate carboxylase/oxygenase enzyme which deliver into the carboxysome and the ssrA degradation tag which is a targeting sequence for degradation by the protease ClpXP. This construct is used as a reporter for the correct assembly of the Carboxysome, it works with the competition of the two targeting sequence, in the case of a correct assembly of the carboxysome the microcompartment will “protect†the fluorescens, in any other case this will be degraded by the protease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None