Generator

Part:BBa_K1113411

Designed by: Belen Cespedes   Group: iGEM13_UC_Chile   (2013-09-18)


Targeting sequence for the delivery of the LacZ gene to the Carboxysome

Includes the lacZ gene (BBa_I732005) which encodes for the β-galactosidase attached with a linker (BBa_K105012) to the large subunit of the RuBisCo (BBa_K1113701) under the regulation of the pBad/araC promoter (BBa_I0500) and includes a RBS (BBa_B0034). The construct is for the delivery of the β-galactosidase and test reactions within the carboxysome. Source of this part: The large subunits of the RuBisCo was obtained from the pHnCBS1D plasmid (plasmid provided by David Savage, Departments of Molecular & Cell Biology and Chemistry, University of California, Berkeley)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2026
    Illegal AgeI site found at 2197
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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Categories
Parameters
None