Generator

Part:BBa_K1113400

Designed by: Sebastin Alvarez   Group: iGEM13_UC_Chile   (2013-09-16)

Targeting sequence for the delivery of the LacZ gene to the Carboxysome.

Includes the lacZ gene which encodes for the β-galactosidase attached with a linker (BBa_K105012) to the large subunit of the RuBisCo under the regulation of the pBad/araC promoter (BBa_I0500) and includes a RBS (BBa_B0034). The construct is for the delivery of the β-galactosidase and test reactions within the carboxysome.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None