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Part:BBa_K1113201

Designed by: Belen Cespedes   Group: iGEM13_UC_Chile   (2013-09-18)


pSB4K5 with a promoter and RBS.

This composite part correspond to the low-copy backbone vector pSB4K5 with the pBad/araC promoter (BBa_I0500) and a RBS (BBa_B0034)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None