Generator

Part:BBa_K1113002

Designed by: Belen Cespedes   Group: iGEM13_UC_Chile   (2013-09-18)

Targeting sequence for the delivery of GFP to the Carboxysome

This targeting sequence consist in the large subunit of the ribulose-1,5-bisphosphate carboxylase/oxygenase enzyme (BBa_K1113701) attached with a flexible linker (BBa_K105012) to the superfolder GFP (BBa_I746916) under the regulation of the pBad/araC promoter (BBa_I0500) and includes a RBS (BBa_B0034). The targeting sequence it’s constructed to deliver the fluorescent to the carboxysome under the induction with arabinose

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2026
    Illegal AgeI site found at 2197
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 2712


[edit]
Categories
Parameters
None