Ice Nucleation Protein fused to Streptavidin Dead
It is a fusion protein between an Ice Nucleation Protein (INP) and Streptavidin Alive made by Gibson Assembly. INP is an outer membrane protein found in the genome of Pseudomonas syringae, that is recognize by the E.coli protein secretion machinery.
Usage and Biology
This part was designed to express streptavidin on the outer membrane of E.coli.
It serves as a negative control as the dead Streptavidin is though to not bind to biotin or with really decreased affinity.
For further Characterization, refer to the datasheet below.
Sequence and Features
- 10Illegal XbaI site found at 1727
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23Illegal XbaI site found at 1727
- 25Illegal XbaI site found at 1727
Illegal NgoMIV site found at 1036
Illegal AgeI site found at 1691
Illegal AgeI site found at 1742
- 1000COMPATIBLE WITH RFC