Ice Nucleation Protein fused to Streptavidin Alive
It is a fusion protein between an Ice Nucleation Protein (INP) and Streptavidin Alive made by Gibson Assembly. INP is an outer membrane protein found in the genome of Pseudomonas syringae, that is recognized by the E.coli protein secretion machinery, and thus also exported in that organism.
Usage and Biology
This part was designed to express streptavidin on the outer membrane of E.coli in order to attach biotinylated nanoparticles to the cells.
Thus, it can be used in any experiment involving biotin-streptavidin affinity (attachment of cargo is one example, but we could also think of purification etc...).
For further Characterization, refer to the datasheet below.
Sequence and Features
- 10Illegal XbaI site found at 1727
- 12COMPATIBLE WITH RFC
- 21Illegal BamHI site found at 1649
- 23Illegal XbaI site found at 1727
- 25Illegal XbaI site found at 1727
Illegal NgoMIV site found at 1036
Illegal AgeI site found at 1691
Illegal AgeI site found at 1742
- 1000COMPATIBLE WITH RFC