Plasmid

Part:BBa_K1110002

Designed by: Lydia Studdard   Group: iGEM13_Georgia_State   (2013-09-17)


Mambalgin cDNA in BBA-K1110001

Plasmid pGAPzalphaC with standardized linker and mamblagin cDNA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 765
    Illegal PstI site found at 944
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 765
    Illegal PstI site found at 944
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 765
    Illegal BglII site found at 1
    Illegal BamHI site found at 1362
    Illegal XhoI site found at 736
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 765
    Illegal PstI site found at 944
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 765
    Illegal PstI site found at 944
    Illegal NgoMIV site found at 2119
    Illegal NgoMIV site found at 2180
    Illegal AgeI site found at 1081
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1345
    Illegal BsaI.rc site found at 1544
    Illegal BsaI.rc site found at 2541
    Illegal SapI site found at 244


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