Plasmid_Backbone

Part:BBa_K1110000

Designed by: Lydia Studdard   Group: iGEM13_Georgia_State   (2013-09-17)


pGAPzalphaA+RFP

Plasmid pGAPzalpha A standardized according to iGEM standards, with RFP sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3113
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3119
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3113
    Illegal BglII site found at 2354
    Illegal BamHI site found at 434
    Illegal XhoI site found at 3089
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3113
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3113
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3128
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1191
    Illegal NgoMIV site found at 1252
    Illegal AgeI site found at 153
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 417
    Illegal BsaI.rc site found at 616
    Illegal BsaI.rc site found at 1613
    Illegal SapI site found at 2597


[edit]
Categories
Parameters
None