Coding

Part:BBa_K1109007

Designed by: Berkhan GENÇ   Group: iGEM13_Baskent_Meds   (2013-10-03)


FepA L8T Mutant -composite of Large Diffusion pore for E. coli outer membrane coding sequence transc

This region contains febA L8T protein which is responsible from diffusion of synthesized proteins through cell membrane to outside of cell. It is transcribed with constitutive promoter. By this means, diffusion of proteins which are in periplasmic space is provided by generating pores on the outer membrane of the double layered membrane structure

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 299
    Illegal AgeI site found at 665
    Illegal AgeI site found at 1292
    Illegal AgeI site found at 1810
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None