Translational_Unit

Part:BBa_K1109006:Design

Designed by: Berkhan GENÇ   Group: iGEM13_Baskent_Meds   (2013-09-23)


lqs response unite


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 549
    Illegal PstI site found at 2707
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 549
    Illegal PstI site found at 2707
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 549
    Illegal PstI site found at 2707
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 549
    Illegal PstI site found at 2707
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2818


Design Notes

The LqsR and LqsS genes have illegal restriction enzyme cutting sites inside their coding regions. So, BBa_K1109002 composite part was amplified with primers having SpeI cutting sites, and cloned into the downstream of a constiutive promoter in the pSB1C3.


Source

Source of the subparts are the 2013 DNA Distribution Kit and pNT-1 plasmid(Cellular Microbiology 9(12):2903-2920, 2007.)

References