Reporter

Part:BBa_K1106006

Designed by: Inés Lucía Patop   Group: iGEM13_Buenos_Aires   (2013-09-09)

Pulse generator part 3

This final construction consist of two independent units:

  • mRFP under a Hybrid pomoter (HSL-LuxR activated, P22 C2 repressed)
  • luxr generator under a constitutive promoter


Usage and Biology

This is a composite part used in our model to generate an incoherent feedforward.

This parts was originally built to be used in iGEM BsAs 2013 project to implement an arsenic sensor device based on synbio technology. Visit our wiki to read details about the designing process and implementation details of our system. Also feel free to contact us for any question regarding this part. Igem.bsas.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 953
    Illegal NheI site found at 976
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 650
    Illegal AgeI site found at 762
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None