GFP generator under Ars promoter
GFP generator under the control of an arsenite sensible promoter.
Usage and Biology
It can be used to quantify arsenic concentration in liquid samples by fluorescense measurement.
|This parts was originally built to be used in iGEM BsAs 2013 project to implement an arsenic sensor device based on synbio technology. Visit our wiki to read details about the designing process and implementation details of our system. Also feel free to contact us for any question regarding this part.|
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 255
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 1191
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Group: NCTU_Formosa 2017
Author: Yi-Ting Lin, Huei-Yu Yeh, Sheng-Ping Chu
New improved part Improvement
BBa_J33201 is a promoter containing the E. coli JM109 chromosomal arsenic detoxification operon (ars operon). Both 2013 Buenos Aires iGEM team and 2006 Edinburgh iGEM team used it to detect arsenic ions in the water. Buenos Aires team ligased it with GFP and Edinburgh team ligased it with LacZ Î±. The two teams also submitted biobricks, BBa_K1106004 and BBa_J33203. We wanted to know whether these two biobricks have more application, such as detecting the arsenic pollution in Chinese medicine. We also wanted to know whether they can detect other metal ions. Several experiments were done to evaluate the function of this promoter.