Composite

Part:BBa_K1104702

Designed by: Po-Hsi Lin   Group: iGEM13_NYMU-Taipei   (2013-10-27)


Positive feedback produced by T7 system

We created the circuit below. Constitutive promoter J23102 is cloned in the head of circuit. With reporter succeeding to the promoter, we could figure out whether the result was caused by promoter or not. And then there is the terminator we would test. After the terminator is the T7 polymerase which would open up the subsequent positive feedback. Besides, we used the other fluorescence protein as our second reporter. By testing the fluorescence from the second reporter, we could know how much proteins would be expressed on this site. Our control is the similar circuit without positive feedback below.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3611
    Illegal NheI site found at 3634
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3308
    Illegal AgeI site found at 3420
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4312


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Categories
Parameters
None