Regulatory

Part:BBa_K110008

Designed by: James DiCarlo   Group: iGEM08_Johns_Hopkins   (2008-05-30)

A-Cell Promoter MFA1

This promoter was isolated by taking 500bp upstream of the MFA1 gene of S. cerevisiae, which encodes for the a-factor pheromone produced by MATa cells. This promoter will be activated when α-factor is expressed in an α haploid yeast cell.

Usage and Biology

The mating pheromone a-factor, made by a cells that MFA1 encodes for will bind to the STE3 G protein-coupled receptor on α cells to initiate shmooing, cell cycle arrest, and mating between the two different cell types.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/eukaryote/yeast
//direction/forward
//chassis/eukaryote/yeast
//promoter
//regulation/unknown
Parameters
negative_regulators-1
positive_regulators-1