Part:BBa_K110005

Alpha-Cell Promoter MF(ALPHA)2

This promoter was isolated by taking 500bp upstream of the MFα2 gene of S. cerevisiae, which encodes the α-factor mating-type α cells. This promoter will be activated when a factor is expressed in an a haploid yeast cell. This is the same sequence as the MFα2 promoter(BBa_K11005), but with suffix and prefix switched (right to left).

Usage and Biology

The MFα2 gene encodes the Mating factor α pheromone. This pheromone is secreted into the surrounding media and it induces shmoo formation, and mating in MAT-a haploid yeast cells. This promoter should be strong if used in haploid α-cells S. cerevisiae cells

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]