Terminator

Part:BBa_K110003

Designed by: James DiCarlo   Group: iGEM08_Johns_Hopkins   (2008-05-30)

Between-SWP82-W and EMP47-C

This single sequence can be used to terminate transcription for two genes in which the first gene (from left to right) is on the Watson strand and the second (from right to left) is on the Crick strand. It was isolated by identifying pairs of nearby convergent genes. The bidirectional terminator is defined as the sequence lying between two convergently oriented ORFs and extending 200 bp into each ORF.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 135
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None