Terminator

Part:BBa_K110001

Designed by: James DiCarlo   Group: iGEM08_Johns_Hopkins   (2008-05-30)


Between-bud 27-W FRS2-C

This terminator can be used for two genes in which the first gene (from left to right) is on the watson strand and the second is on the crick strand. It was isolated by finding an UTR between two close W-C orented genes and going 200 bp into both genes it flanked.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 498
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 17
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None