Part:BBa_K1097000

adh2（Saccharomyces cerevisiae）

Overview

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Our part is a coding sequence of ADH2 from Saccharomyces cerevisiae. It codes an enzyme which converts alcohol to corresponding aldehyde. You simply insert this part into an expression vector like pET-28a(+) (Novagen), and the corresponding enzyme yeast ADH2 can be expressed under suitable condition. This enzyme preferentially catalyzes the conversion of ethanol to acetaldehyde and can act on a variety of primary unbranched aliphatic alcohols.

Function

This enzyme preferentially catalyzes the conversion of ethanol to acetaldehyde and it can act on a variety of primary unbranched aliphatic alcohols.

Source

Faculty of Technology, The University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Yugoslavia. leskovac@tehnol.ns.ac.yu

Structure

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

[1]Leskovac V, Trivic S,and Pericin D (2002). The three zinc-containing alcohol dehydrogenases from baker's yeast, Saccharomyces cerevisiae. FEMS Yeast Res. 2: 481-494.

[2]Russell DW et al (1983). Nucleotide sequence of the yeast alcohol dehydrogenase II gene. J Biol Chem. 258(4): 2674-82.

[3]Bowman S et al (1997). The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII. Nature, 387: 90-3.

[4]Garrels JI et al (1997). Proteome studies of Saccharomyces cerevisiae: identification and characterization of abundant proteins. Electrophoresis. 18(8): 1347-60.

[5]Zhao Q et al (2010). Characterization of Alcohol Dehydrogenase from Permeabilized Brewer's Yeast Cells Immobilized on the Derived Attapulgite Nanofibers. Applied Biochemistry and Biotechnology,160:2287-2299.

[6]Zanon JP et al (2007). Colorimetric assay of ethanol using alcohol dehydrogenase from dry baker’s yeast. Enzyme and Microbial Technology.3(40): 466-470.

[http://www.ncbi.nlm.nih.gov/nuccore/NM_001182812.1 NCBI_ID]