Coding

Part:BBa_K109700

Designed by: Ingrid Swanson   Group: iGEM08_University_of_Washington   (2008-07-28)


lac permease in yeast

this ORF encodes permease in the cell wall of K. lactis that imports lactose into the cytoplasm allowing metabolism and growth on lactose. Can be used in K. lactis, S. cerevisae, etc. Must be paired a beta-galactosidase to allow lactose metabolism.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 881
    Illegal BamHI site found at 744
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None