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Part:BBa_K1093023

Designed by: Jakub Piątkowski   Group: iGEM13_Warsaw   (2013-09-23)


MinC/Lysis device-based kill switch

This brick combines elements from the well described bacterial lysis device (BBa_K1120808) with the gene coding for MinC protein (cell division inhibitor, characterised by Team Warsaw 2010). Addition of MinC is meant do reduce the odds of individual cells adapting to the device, thus making it suitable for the programmed cell death of bacterial population. System is under the control of BBa_K098995 heat-inducible input device. The kill-switch is activated in 42 degrees Celsius.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 3067
    Illegal NheI site found at 3090
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1224
    Illegal AgeI site found at 2672
    Illegal AgeI site found at 2742
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3323


[edit]
Categories
Parameters
None