Device

Part:BBa_K1092025

Designed by:   Group: iGEM13_Hong_Kong_CUHK   (2013-09-18)

T7-RBS -ssDsbA-PDZ Ligand-Voltage Switch-Laccase

This biobrick is the counterpart of the part: T7-RBS-ssDsbA-PDZ Domain-Voltage sensor peptide-Dioxygenase (BBa_K1092026), and is composed of the voltage switch (BBa_K1092009 & BBa_K1092010) and the Dioxygenase (BBa_K1092002) and Laccase (BBa_K1092004). In this example, we explore the possiblity of using our novel voltage switch as a fast control of reaction rate. We made use of the Laccase and Dioxygenase as an example to illustrate this.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 929
    Illegal AgeI site found at 2447
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 550
    Illegal BsaI.rc site found at 2608


[edit]
Categories
Parameters
None