Coding

Part:BBa_K1091004

Designed by: Jian Huang   Group: iGEM13_UESTC   (2013-09-17)

pBAD-green fluorescent protein-FAST

Part K1091004 is one of the CDS parts designed with the Transpeeder software version 1.0. Though it has an identical amino acid sequence with the wild type green fluorescent protein, its nucleic sequence is designed to show less similarities with the Shine-Dalgarno sequence of E.coli via synonymous mutation using Transpeeder. The preliminary experimental results showed it had a faster translation speed than those of the wild type (K1091006) and slow parts (K1091005). Thus, the part is also called GFP-fast. Pay attention! This part is cloned in pBAD plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 664
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 664
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 664
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 664
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 664
    Illegal NgoMIV site found at 679
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None