Coding

Part:BBa_K1091002

Designed by: Jian Huang   Group: iGEM13_UESTC   (2013-09-17)

green fluorescent protein－slow

Part K1091002 is one of the CDS parts designed with the Transpeeder software version 1.0. Though it has an identical amino acid sequence with the wild type green fluorescent protein, its nucleic sequence is designed to show more similarities with the Shine-Dalgarno sequence of E.coli via synonymous mutation using Transpeeder. The preliminary experimental results using its twin part K1091005 showed it had a slower translation speed than those of the wild type and fast parts.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 627
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None