Coding
Part:BBa_K1091001
Designed by: Jian Huang Group: iGEM13_UESTC (2013-09-17)
green fluorescent protein with a fast translation speed
Part K1091001 is one of the CDS parts designed with the Transpeeder software version 1.0. Though it has an identical amino acid sequence with the wild type green fluorescent protein, its nucleic sequence is designed to show less similarities with the Shine-Dalgarno sequence of E.coli via synonymous mutation using Transpeeder. The preliminary experimental results using its twin part K1091004 showed it had a faster translation speed than those of the wild type and slow parts.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 664
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 664
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 664
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 664
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 664
Illegal NgoMIV site found at 679 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |