Regulatory
pLac

Part:BBa_K1088054

Designed by: Patrick Rosendahl Andreassen   Group: iGEM13_SDU-Denmark   (2015-03-30)


Lac operator

This part is an inverting regulator sensitive to LacI and CAP.

It contains two protein binding sites. The first binds the CAP protein, which is generally present in E.coli and is asocciated with cell health and availability of glucose. The second binds LacI protein.

In the absence of LacI protein and CAP protein, this part promotes transcription. In the presence of LacI protein and CAP protein, this part inhibits transcription. LacI can be inhibited by IPTG. LacI is coded by BBa_C0010

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None