Part:BBa_K1088052

GFP reporter with flexible linker at N-terminus for creation of GFP fusions

This part will be suffixed to proteins to create protein-GFP fusions with a flexible linker in between to increase chances of both proteins to be functional.

GFP fusions have variuos functions e.g. protein localization assays, immunoblotting and protein purification using anti-GFP antibodies. For protein purification, or other applications of seperating the fused proteins, a TEV protease recognition site is included in the flexible linker.

Note that standard RFC[10] assembly with a part suffixed to this part will result in a scar-site that includes an unwanted stop-codon. To prevent this, PCR amplification of inserts using appropriate primers is to be employed (see design page for details)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 674