Protein_Domain
Linker

Part:BBa_K1088051

Designed by: Patrick Rosendahl Andreassen   Group: iGEM13_SDU-Denmark   (2015-03-16)


10 aa linker with BamHI restriction site and TEV recognition site

A flexible 10 amino acid linker that enables for functional protein/domain fusions.

A BamHI site is included in order to prevent creation of a scarsite (in-frame scarsites encode stop-codons). Assembly of parts therefore has to be done with PCR amplification of inserts using appropriate primers instead of standard RFC[10] assembly of parts (see design page for further details).

The encoded peptide (GSENLYFQSG) includes a recognition site for the TEV protease (ENLYFQS) that enables for seperation of the fused proteins/domains.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None