Device

Part:BBa_K1088012

Designed by: Andreas Kjr   Group: iGEM13_SDU-Denmark   (2013-08-28)

E. coli dxs (lac promoter without lac inhibitor)

This part contains the dxs gene derived from E. coli (BBa_K118000) controlled by the lac promoter and has a strong RBS. The part was build to increase the flow through the MEP (methylerythritol phosphate) pathway, and to test whether Dxs derived from E. coli or B. subtilis increased the flow through the MEP pathway the most in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 946


[edit]
Categories
Parameters
None